2019-02-08 18:10:142019-02-08 18:06:57
Biological materials
Mutant
MGNA-A328 (hprK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/328 NBRP B. subtilis, Japan]
GP202 (Δ[[gene|hprK]]::spc) [Pubmed|12055300], available in [SW|Jörg Stülke]'s lab
GP858 (Δ[[gene|hprK]]::aphA3pc) [Pubmed|18757537], available in [SW|Jörg Stülke]'s lab
GP82 (Δ[[gene|hprK]]::cat) [Pubmed|21992469], available in [SW|Jörg Stülke]'s lab
BKE35000 (Δ[[gene|hprK]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGATATGTTCCTCCTGTT, downstream forward: _UP4_CAAGAAGAATAGGAGTTGGC
BKK35000 (Δ[[gene|hprK]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGATATGTTCCTCCTGTT, downstream forward: _UP4_CAAGAAGAATAGGAGTTGGC
MGNA-A328 (yvoB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/328 NBRP B. subtilis, Japan]
GP202 (spc) [Pubmed|12055300], GP858 (aphA3) [Pubmed|18757537], GP82 (cat) [Pubmed|21992469], all available in [SW|Jrg Stlke]'s lab
BKE35000 ([[gene|hprK]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGATATGTTCCTCCTGTT, downstream forward: _UP4_CAAGAAGAATAGGAGTTGGC
BKK35000 ([[gene|hprK]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGATATGTTCCTCCTGTT, downstream forward: _UP4_CAAGAAGAATAGGAGTTGGC
Biological materials
Expression vectors
for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP642, available in [SW|Jörg Stülke]'s lab
for expression/ purification of mutant HprK-G158A from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP650, available in [SW|Jörg Stülke]'s lab
for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP205, available in [SW|Jörg Stülke]'s lab
for expression, purification of the N-terminal in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP218, available in [SW|Jörg Stülke]'s lab
for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP642, available in [SW|Jrg Stlke]'s lab
for expression/ purification of mutant HprK-G158A from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP650, available in [SW|Jrg Stlke]'s lab
for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP205, available in [SW|Jrg Stlke]'s lab
for expression, purification of the N-terminal in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP218, available in [SW|Jrg Stlke]'s lab
Biological materials
lacZ fusion
pGP201 (in [[protein|pAC5]], [Pubmed|12055300]) available in [SW|Stlke] lab, pGP202 (in [[protein|pAC6]], [Pubmed|12055300]) available in [SW|Jörg Stülke]'s lab
pGP201 (in [[protein|pAC5]], [Pubmed|12055300]) available in [SW|Stlke] lab, pGP202 (in [[protein|pAC6]], [Pubmed|12055300]) available in [SW|Jrg Stlke]'s lab
Biological materials
Antibody
available in [SW|Jörg Stülke]'s lab [Pubmed|12055300]
available in [SW|Jrg Stlke]'s lab [Pubmed|12055300]
labs
[SW|Jörg Stülke], University of Gttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
[SW|Anne Galinier], University of Marseille, France
[SW|Josef Deutscher], Paris-Grignon, France
[SW|Jrg Stlke], University of Gttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
[SW|Wolfgang Hillen], Erlangen University, Germany [http://www.biologie.uni-erlangen.de/mibi/index2.html Homepage]
[SW|Anne Galinier], University of Marseille, France